Videogrammetric monitoring of as-built membrane roof structures     

Shih-Yuan  Lin  Jon P.  Mills  Peter D.  Gosling 《The Photogrammetric Record》2008,23(122):128-147

A monitoring system that uses applied geomatics techniques, including videogrammetry and terrestrial laser scanning, has been developed in order to investigate the dynamic behaviour of as-built membrane roof structures. To accommodate the problems involved in measuring such roof structures, examples of which include uniform surface texture and unreachable height, the system adopts a non-contact targeting methodology to substitute for traditional control techniques. In-house software was developed for processing the acquired video imagery in order to produce a sequential surface model of the membrane. This paper firstly reports on experiments conducted to demonstrate that the monitoring system was capable of measuring representative test structures with a precision of better than ±1 mm. To prove the feasibility and transferability of the system, videogrammetric monitoring campaigns were subsequently performed at a real-world test site where two different types of membrane structure were observed. The sequential 3D surface models that were generated allowed the displacement of the membrane surfaces to be investigated over a period, during which the range of the movement reached over 20 mm. The information acquired from the system is proving to be of great value to structural engineers involved in research and development of such membrane structures.  相似文献   

Benthic fluxes of nitrogen in the tidal reaches of a turbid, high-nitrate sub-tropical river     

Perran L. M. Cook  Bradley D. Eyre  Rhys Leeming  Edward C. V. Butler   《Estuarine, Coastal and Shelf Science》2004,59(4):675-685

Benthic fluxes of dissolved inorganic nitrogen (NO₃⁻ and NH₄⁺), dissolved organic nitrogen (DON), N₂ (denitrification), O₂ and TCO₂ were measured in the tidal reaches of the Bremer River, south east Queensland, Australia. Measurements were made at three sites during summer and winter. Fluxes of NO₃⁻ were generally directed into the sediments at rates of up to −225 μmol N m⁻² h⁻¹. NH₄⁺ was mostly taken up by the sediments at rates of up to −52 μmol N m⁻² h⁻¹, its ultimate fate probably being denitrification. DON fluxes were not significant during winter. During summer, fluxes of DON were observed both into (−105 μmol m⁻² h⁻¹) and out of (39 μmol m⁻² h⁻¹) the sediments. Average N₂ fluxes at all sampling sites were similar during summer (162 μmol N m⁻² h⁻¹) and winter (153 μmol N m⁻² h⁻¹). Denitrification was fed both by nitrification within the sediment and NO₃⁻ from the water column. Sediment respiration rates played an important role in the dynamics of nitrification and denitrification. NO₃⁻ fluxes were significantly related to TCO₂ fluxes (p<0.01), with a release of NO₃⁻ from the sediment only occurring at respiration rates below 1000 μmol C m⁻² h⁻¹. Rates of denitrification increased with respiration up to TCO₂ fluxes of 1000 μmol C m⁻² h⁻¹. At sediment respiration rates above 1000 μmol C m⁻² h⁻¹, denitrification rates increased less rapidly with respiration in winter and declined during summer. On a monthly basis denitrification removed about 9% of the total nitrogen and 16% of NO₃⁻ entering the Bremer River system from known point sources. This is a similar magnitude to that estimated in other tidal river systems and estuaries receiving similar nitrogen loads. During flood events the amount of NO₃⁻ denitrified dropped to about 6% of the total river NO₃⁻ load.  相似文献   

致病性鳗弧菌W-1外膜蛋白ompU基因克隆及在大肠杆菌中的表达   总被引：1，自引：1，他引：1  

杨慧  陈吉祥  公衍军  李彩风  李筠  杨官品 《中国海洋大学学报(自然科学版)》2006,36(Z1):105-108

从致病性鳗弧菌W-1基因组DNA扩增并克隆了1 156bp的特异性片段,含有完整的外膜蛋白ompU基因阅读框,由993个核苷酸组成,编码330个氨基酸残基的蛋白质。与国外已发表的鳗弧菌外膜蛋白基因的序列同源性为100%,与创伤弧菌(Vibrio vulnificus)、霍乱弧菌(Vibrio cholerae)、费氏弧菌(Vibrio fischeri)和副溶血弧菌(Vibrio parahaemolyticus)的序列同源性分别为72%,69%,68%和64%。将该外膜蛋白基因克隆于pBV220表达质粒,在大肠杆菌中得到了表达,SDS-PAGE分析表明表达蛋白分子量约38kDa,Western blot分析发现表达蛋白能与鳗弧菌外膜蛋白抗体很好的反应。  相似文献   

基于聚二甲基硅氧烷胶与Zn-Al型水滑石复合膜为离子载体的溶解无机碳电极及其性能标定          下载免费PDF全文

韩沉花  潘依雯  叶瑛 《热带海洋学报》2009,28(4):35-41

深海探测溶解无机碳含量异常,可以为寻找热液口提供一个很好的证据。本文设计的基于自制的聚二甲基硅氧烷胶与Zn-Al型水滑石复合膜为离子载体的溶解无机碳探测电极属于电势型电极,适用于对深海中溶解无机碳的测量,具有体积微小、便于与pH、盐度、温度、氧化还原电位等多种传感器集成在同一探头腔内,实施在线探测等优点。实验室条件下,将该溶解无机碳探测电极与银/氯化银参比电极分别接在Keithley 6517A高阻抗电位计输入端进行测试,测试结果表明：该电极对不同浓度的碳酸氢钠溶液具有良好的响应信号,响应时间大约在30s—1 min,检测限在10－1—10－4mol?L–1。  相似文献   

光纤光化学氨传感器的研究及应用   总被引：2，自引：0，他引：2  

戴媛静  陈曦  庄峙厦  李伟  王小如 《海洋技术学报》2003,22(2):37-43

氨是导致海洋水体富营养化的重要因素之一，基于光纤光化学检测的氨传感器特别适于氨的原位或在线分析。文章介绍了光纤光化学氨传感器的基本原理，光纤敏感膜的制备方法及其一些应用管况。发展小型、实用的光纤光化学传感器是未来海水中氨的原位、在线检测的发展趋势之一。  相似文献   

银杏叶提取物(EGb761)对红细胞脂质过氧化损伤的影响   总被引：6，自引：0，他引：6  

李静  刘成玉 《中国海洋大学学报(自然科学版)》2005,35(3):487-490,381

观察银杏叶提取物(EGb761)对红细胞脂质过氧化损伤的影响,探讨其保护红细胞的机制。以活性氧(H2 O2 )作用于离体的正常红细胞,观察EGb761对红细胞溶血度、红细胞丙二醛含量(MDA)、红细胞超氧化物歧化酶(SOD)和ATP酶(ATPase)活性及红细胞膜骨架结构的影响。结果表明:EGb761能明显降低红细胞溶血度,且随着浓度增加其作用逐渐增强,与对照组比较差异有极显著性(t =4.13～19.41,P <0 .0 5 ,0 .0 0 1) ;EGb761能明显降低红细胞MDA含量,提高其SOD ,ATPase酶活性,与对照组比较差异有极显著性(t =2 .0 2～70 .0 6,P <0 .0 5 ,0 .0 0 1) ;EGb761能降低过氧化损伤对红细胞膜骨架的破坏,保护其完整性。可见EGb761能在膜水平上直接对抗氧自由基引发的红细胞脂质过氧化损伤,保护红细胞及其膜骨架结构。  相似文献   

Degradation of outer membrane proteins of <Emphasis Type="Italic">Synechococcus</Emphasis> sp. <Emphasis Type="Italic">in vitro</Emphasis> and <Emphasis Type="Italic">in situ</Emphasis>     

Tatsuo?Miyoshi  Satoru?SuzukiEmail author 《Journal of Oceanography》2004,60(5):825-833

To examine the possibility that outer membrane proteins (OMP) of Synechococcus sp. remain in seawater, we investigated the stability of OMPs in vitro and in situ. Some fractions prepared from Synechococcus sp. CSIRO-94 were treated with trypsin and proteinase K. Four tightly bound OMPs were separated from Synechococcus. We designated the two major OMPs of 52 kDa and 48 kDa as Omp52Sy and Omp48Sy, respectively. Degradation of the OMP in natural seawater was monitored in microcosms to which intact Synechococcus cells and outer membrane (OM) were added. Omp52Sy and Omp48Sy were the most stable against trypsin and proteinase K among the OMPs when they were embedded in the OM. However, in the microcosm experiment using intact cells, Omp52Sy and Omp48Sy were detected in the particulate fraction only during the first 4 days, after which they could not longer be detected. Omp52Sy and Omp48Sy were the most stable proteins among the Synechococcus OMPs in vitro, but they might be degraded in situ. This indicates that stability of Synechococcus porin differs depending on complex formation with other membrane molecules, which might cause different preservation of microbial membrane proteins in the dissolved protein pool in the ocean. This study suggests that Gram negative bacterial OM with thin peptidoglycan forms a lipid bilayer that proptects OMP, but Synechococcus OM with thick peptidoglycan cannot form a lipid bilayer. The incomplete bilayer might not be able to protect from protease attack in the natural environment.  相似文献   

防渗膜渗漏偶极子检测的应用     

杨萍  孔波  姜余祥  王燕妮 《物探与化探》2014,(6)

偶极子法可用于防渗膜渗漏检测。膜上介质厚度以及偶极子与膜的距离会对偶极子检测的灵敏度产生影响。基于填埋场防渗膜高压直流电法的检测原理,对膜上介质厚度对偶极子检测的影响进行了研究,结果表明:防渗膜上介质厚度增大,通过在介质表面上移动偶极子来检测漏洞是困难的;但若偶极子能逼近膜的表面进行检测,无论膜上介质厚度多大,在漏洞附近都能检测到明显的电势异常。  相似文献   

边界问题及其对真空预压的影响     

高志义  冯军 《岩土工程技术》2014,28(5):250-256

给出了真空预压区四周开敞式与封闭式边界定义、两者的不同,分析了开敞式边界加固效果优于封闭式边界的原因并以三个实例说明之,给出如何将封闭式转化为开敞式的方法;柔性与刚性密封膜同样可构成开敞式与封闭式边界,讨论了此时两种边界下差别;给出了密封墙和支挡墙的定义、功能、应用范围;讨论了水泥搅拌与旋喷桩单体墙与格栅墙的定义、功能、应用范围,前者只能作为密封墙,后者既可作密封墙也可作支挡墙.  相似文献   

壳聚糖超滤复合膜研制   总被引：1，自引：0，他引：1  

孙秀珍  张碧珍 《海洋学研究》1999,17(2):21-25

以聚醚砜（ＰＥＳ）、聚偏氟乙烯（ＰＶＤＦ）超滤膜、聚己内酰胺（尼龙－６）微孔滤膜为多孔支撑膜,将天然聚合物壳聚糖通过流涎法制成超滤复合膜。该复合膜性能优良,在操作压力为０．２５ＭＰａ的条件下,它对聚乙二醇（ＰＥＧ）的截留率及其渗透速度,分别为８７．２％～９８．４％和２．１～５９．０Ｌ／（ｍ２ｈ）。壳聚糖超滤复合膜性能的优劣与支撑膜的材质、孔径、壳聚糖的浓度、添加剂的分子量及成膜时的条件有关。  相似文献